Intradermal skin testing for food allergies may not give an accurate diagnosis in every dog. This test can give false positive and false negative results. In a false positive situation, despite the fact the dog is not allergic to the food when ingested, it results in a positive allergic skin response. As for a false negative, some food allergies produce a delayed result of a positive allergic reaction. This causes the veterinarian to believe your dog is not allergic to the allergen since she didn’t see a skin response, but in reality, he is allergic to it.
Serology can be run to check for antibody levels. As with intradermal testing, results aren't always accurate but it can be helpful.
Bicom testing is an unproven method that claims to be able to detect allergies, which you may have heard of. Bicom testing is also known as bioresonance. This method believes every being and substance in the world emits its own electromagnetic wave. The veterinarian takes a blood sample from your dog and tests different food items and substances to see if it has a ‘good’, ‘neutral’ or ‘bad’ response. If the response is ‘bad’, the item being tested causes your dog’s blood wavelength to become stressed. Stressed blood leads to a stressed body and therefore an allergic reaction. If the response is ‘good’, then the item puts out a wavelength that is compatible with your dog’s blood which means no adverse reactions should occur. If it is ‘neutral’, the item being tested does not put out a wavelength that alters that of your dog. Most vets will not offer this service as there is no data which backs it up.
A dietary trial is the most frequently used method of diagnosis. In a dietary trial, you have to feed your dog a novel diet, meaning you feed him a diet without any fish ingredients whatsoever. During this trial, you cannot give any type of treats containing fish or fish flavored medications as it can affect the results. You have to feed your dog the novel diet for at least 90 days before you can get a reliable diagnostic. After the 90 days, if resolution of the allergic symptoms occurs, you then must reintroduce fish to his diet. If a relapse in allergy symptoms occurs once the fish is reintroduced, then you know the source. If symptoms do not return with the reintroduction of fish, then it was a source other than the fish your dog was reacting to.